|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
24/10/2023 |
Actualizado : |
24/10/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BRANDA-SICA, A.; NICOLINI, M.P.; ARTIGAS, R.; FEDERICI, M.; LLAMBÍ, S. |
Afiliación : |
ANDREA BRANDA SICA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA PAULA NICOLINI DUARTE, Universidad de la República, Centro Universitario de Tacuarembó, Instituto Superior de la Carne, Área Biología Molecular. Tacuarembó, Uruguay; RODY ARTIGAS, Universidad de la República, Facultad de Veterinaria, Unidad Académica de Genética y Mejora Animal. Montevideo, Uruguay; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA LLAMBÍ, Universidad de la República, Facultad de Veterinaria, Unidad Académica de Genética y Mejora Animal. Montevideo, Uruguay. |
Título : |
Optimización de PCR en tiempo real con curvas de disociación para la detección de la mutación causante de deficiencia de colesterol en bovinos Holando. [Optimization of Real-Time PCR-melting for detection of the Cholesterol-deficiency mutation in Holstein Friesian cattle]. |
Complemento del título : |
Sección Medicina Veterinaria. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Revista Cientifica Facultad de Ciencias Veterinarias, 2022, volume 32, e32137, 1-5. https://doi.org/10.52973/rcfcv-e32137 -- OPEN ACCESS. |
ISSN : |
0798-2259 (print); 2521-9715 (electronic). |
DOI : |
10.52973/rcfcv-e32137 |
Idioma : |
Inglés |
Notas : |
Article history: Received 15 March 2022, Accepted 30 March 2022, Published 21 June 2022. -- Correspondence author: Andrea Branda, email: abranda@inia.org.uy -- LICENSE: Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0. (https://creativecommons.org/licenses/by-nc-sa/4.0/ ). |
Contenido : |
RESUMEN: El objetivo de este estudio fue optimizar un análisis mediante PCR en tiempo real con curvas de disociación para la detección confiable y económica del inserto mutante de 7,5 Kb del elemento transponible bovino BoERVK en el exón 5 del gen de la Apolipoproteína B (APOB), determinante de la deficiencia de colesterol - CD - (OMIA 001965-9913). Asimismo, aplicando esta técnica se realizó un cribado molecular preliminar para determinar la presencia de esta mutación en una muestra de ADN de vacas Holando (H) pertenecientes a seis tambos o fincas comerciales de diferentes regiones del Uruguay. A partir de la amplificación de los productos de PCR de 170 y 146 pb se logró distinguir claramente dos genotipos: homocigota (tipo silvestre wt/wt) y heterocigota (portador de la mutación CD: MUT/wt). El genotipo homocigota wt/wt fue detectado en la muestra representativa de 103 vacas H. Se concluye que el análisis mediante PCR en tiempo real con curvas de disociación es una técnica rápida, fácilmente interpretable, de bajo costo y altamente precisa para la detección de esta mutación, el cual puede ser implementado en programas de selección genética para evitar la propagación de la enfermedad en bovinos H. ------- ABSTRACT: The purpose of this study was to optimize a real-time PCR-melting analysis for reliable and economical detection of the 7.5 Kb mutant insert of the BoERVK bovine transposable element in exon 5 of the Apolipoprotein B (APOB) gene, which causes cholesterol deficiency - CD - (OMIA 001965-9913). This technique was also used to perform a preliminary molecular screening to detect this mutation in a DNA sample of Holstein Friesian cows (HFc) of six commercial dairy farms from different regions of Uruguay. By amplifying the 170 and 146 bp PCR products, two genotypes were clearly identified: homozygote (wild type wt/wt) and heterozygote (carrier of the CD mutation: MUT/wt). The homozygous wt/wt genotype was detected in the representative sample of 103 HFc. It is concluded that Real-Time PCR-melting analysis is a fast, easily interpretable, low cost, and highly accurate technique for detecting this mutation, which can be implemented in genetic selection programs to prevent the spread of the disease in HFc. MenosRESUMEN: El objetivo de este estudio fue optimizar un análisis mediante PCR en tiempo real con curvas de disociación para la detección confiable y económica del inserto mutante de 7,5 Kb del elemento transponible bovino BoERVK en el exón 5 del gen de la Apolipoproteína B (APOB), determinante de la deficiencia de colesterol - CD - (OMIA 001965-9913). Asimismo, aplicando esta técnica se realizó un cribado molecular preliminar para determinar la presencia de esta mutación en una muestra de ADN de vacas Holando (H) pertenecientes a seis tambos o fincas comerciales de diferentes regiones del Uruguay. A partir de la amplificación de los productos de PCR de 170 y 146 pb se logró distinguir claramente dos genotipos: homocigota (tipo silvestre wt/wt) y heterocigota (portador de la mutación CD: MUT/wt). El genotipo homocigota wt/wt fue detectado en la muestra representativa de 103 vacas H. Se concluye que el análisis mediante PCR en tiempo real con curvas de disociación es una técnica rápida, fácilmente interpretable, de bajo costo y altamente precisa para la detección de esta mutación, el cual puede ser implementado en programas de selección genética para evitar la propagación de la enfermedad en bovinos H. ------- ABSTRACT: The purpose of this study was to optimize a real-time PCR-melting analysis for reliable and economical detection of the 7.5 Kb mutant insert of the BoERVK bovine transposable element in exon 5 of the Apolipoprotein B (APOB) gene, which causes cholesterol deficien... Presentar Todo |
Palabras claves : |
Cholesterol deficiency; Deficiencia de colesterol; Holstein Friesian; PCR en tiempo real con curvas de disociación; Real-time PCR-melting. |
Thesagro : |
HOLANDO. |
Asunto categoría : |
L50 Fisiología y bioquímica animal |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17399/1/38277-Texto-del-articulo-71597-2-10-20220621.pdf
|
Marc : |
LEADER 03739naa a2200277 a 4500 001 1064357 005 2023-10-24 008 2022 bl uuuu u00u1 u #d 022 $a0798-2259 (print); 2521-9715 (electronic). 024 7 $a10.52973/rcfcv-e32137$2DOI 100 1 $aBRANDA-SICA, A. 245 $aOptimización de PCR en tiempo real con curvas de disociación para la detección de la mutación causante de deficiencia de colesterol en bovinos Holando. [Optimization of Real-Time PCR-melting for detection of the Cholesterol-deficiency mutation in Holstein Friesian cattle].$h[electronic resource] 260 $c2022 500 $aArticle history: Received 15 March 2022, Accepted 30 March 2022, Published 21 June 2022. -- Correspondence author: Andrea Branda, email: abranda@inia.org.uy -- LICENSE: Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0. (https://creativecommons.org/licenses/by-nc-sa/4.0/ ). 520 $aRESUMEN: El objetivo de este estudio fue optimizar un análisis mediante PCR en tiempo real con curvas de disociación para la detección confiable y económica del inserto mutante de 7,5 Kb del elemento transponible bovino BoERVK en el exón 5 del gen de la Apolipoproteína B (APOB), determinante de la deficiencia de colesterol - CD - (OMIA 001965-9913). Asimismo, aplicando esta técnica se realizó un cribado molecular preliminar para determinar la presencia de esta mutación en una muestra de ADN de vacas Holando (H) pertenecientes a seis tambos o fincas comerciales de diferentes regiones del Uruguay. A partir de la amplificación de los productos de PCR de 170 y 146 pb se logró distinguir claramente dos genotipos: homocigota (tipo silvestre wt/wt) y heterocigota (portador de la mutación CD: MUT/wt). El genotipo homocigota wt/wt fue detectado en la muestra representativa de 103 vacas H. Se concluye que el análisis mediante PCR en tiempo real con curvas de disociación es una técnica rápida, fácilmente interpretable, de bajo costo y altamente precisa para la detección de esta mutación, el cual puede ser implementado en programas de selección genética para evitar la propagación de la enfermedad en bovinos H. ------- ABSTRACT: The purpose of this study was to optimize a real-time PCR-melting analysis for reliable and economical detection of the 7.5 Kb mutant insert of the BoERVK bovine transposable element in exon 5 of the Apolipoprotein B (APOB) gene, which causes cholesterol deficiency - CD - (OMIA 001965-9913). This technique was also used to perform a preliminary molecular screening to detect this mutation in a DNA sample of Holstein Friesian cows (HFc) of six commercial dairy farms from different regions of Uruguay. By amplifying the 170 and 146 bp PCR products, two genotypes were clearly identified: homozygote (wild type wt/wt) and heterozygote (carrier of the CD mutation: MUT/wt). The homozygous wt/wt genotype was detected in the representative sample of 103 HFc. It is concluded that Real-Time PCR-melting analysis is a fast, easily interpretable, low cost, and highly accurate technique for detecting this mutation, which can be implemented in genetic selection programs to prevent the spread of the disease in HFc. 650 $aHOLANDO 653 $aCholesterol deficiency 653 $aDeficiencia de colesterol 653 $aHolstein Friesian 653 $aPCR en tiempo real con curvas de disociación 653 $aReal-time PCR-melting 700 1 $aNICOLINI, M.P. 700 1 $aARTIGAS, R. 700 1 $aFEDERICI, M. 700 1 $aLLAMBÍ, S. 773 $tRevista Cientifica Facultad de Ciencias Veterinarias, 2022, volume 32, e32137, 1-5. https://doi.org/10.52973/rcfcv-e32137 -- OPEN ACCESS.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
27/08/2020 |
Actualizado : |
27/01/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
SCHILD, C.; CAFFARENA, D.; GIL, A.; SANCHEZ, J.; RIET-CORREA, F.; GIANNITTI, F. |
Afiliación : |
CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Facultad de Veterinaria, Universidad de la República (UDELAR), 11600, Montevideo, Uruguay.; ANDRÉS GIL, Facultad de Veterinaria, Universidad de la República (UDELAR), 11600, Montevideo, Uruguay.; JAVIER SANCHEZ, Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Prince Edward Island C1A 4P3, Canada.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
A survey of management practices that influence calf welfare and an estimation of the annual calf mortality risk in pastured dairy herds in Uruguay. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Journal of Dairy Science, Volume 103, Issue 10, pag. 9418-9429, October 2020. Doi: https://doi.org/10.3168/jds.2020-18177 |
DOI : |
doi.org/10.3168/jds.2020-18177 |
Idioma : |
Inglés |
Notas : |
Article history: Received January 8, 2020/Accepted May 26, 2020. Corresponding authors: cschild@inia.org.uy
and fgiannitti@inia.org.uy. |
Contenido : |
ABSTRACT: Despite the importance of dairy farming in Uruguay,little information on dairy systems in this country is available in the scientific literature, and management practices that influence calf welfare at the herd level have not been explored. The aims of this study were to
(1) describe the prepartum and calf-rearing systems, as well as the management practices that may influence calf welfare in pastured dairy herds in Uruguay, (2) estimate the annual calf mortality risk from birth to weaning, and (3) identify the primary clinical disease syndromes shown by the calves before death. A survey comprising a farm visit and a questionnaire was conducted on 225 randomly selected dairies with >30
milking cows, in 3 strata (31?99, 100?299, and ?300 milking cows) of 6 Uruguayan departments where dairies are concentrated. Retrospective information from July 2013 to June 2014 was collected. A descriptive analysis was performed and results were inferred into
the national dairy cattle population. Several management practices that could contribute to poor calf welfare were identified in a large proportion of farms. The annual calf mortality risk (calves that died between birth and weaning/calves born death or alive × 100,
n = 149 farms) was 15.2%. Age at weaning averaged 75 d. Farmers reported that the most common clinical syndromes were diarrhea and respiratory disease in 85.2% and 47.5% of the farms, respectively. There was no continuous veterinary advice in 61.3% of the farms, 20.0% lacked data records, 38.5% had poor drainage in the prepartum area with waterlogging after rainfall, 52.1% monitored the prepartum area ?2 times per day MenosABSTRACT: Despite the importance of dairy farming in Uruguay,little information on dairy systems in this country is available in the scientific literature, and management practices that influence calf welfare at the herd level have not been explored. The aims of this study were to
(1) describe the prepartum and calf-rearing systems, as well as the management practices that may influence calf welfare in pastured dairy herds in Uruguay, (2) estimate the annual calf mortality risk from birth to weaning, and (3) identify the primary clinical disease syndromes shown by the calves before death. A survey comprising a farm visit and a questionnaire was conducted on 225 randomly selected dairies with >30
milking cows, in 3 strata (31?99, 100?299, and ?300 milking cows) of 6 Uruguayan departments where dairies are concentrated. Retrospective information from July 2013 to June 2014 was collected. A descriptive analysis was performed and results were inferred into
the national dairy cattle population. Several management practices that could contribute to poor calf welfare were identified in a large proportion of farms. The annual calf mortality risk (calves that died between birth and weaning/calves born death or alive × 100,
n = 149 farms) was 15.2%. Age at weaning averaged 75 d. Farmers reported that the most common clinical syndromes were diarrhea and respiratory disease in 85.2% and 47.5% of the farms, respectively. There was no continuous veterinary advice in 61.3% of the farms, 20... Presentar Todo |
Palabras claves : |
BINESTAR DE LOS TERNEROS; CALF MORTALITY; CALF WELFARE; DAIRY FARMING; ENCUESTA; MORTALIDAD DE TERNEROS; PLATAFORMA SALUD ANIMAL; SURVEY. |
Thesagro : |
PRODUCCION LECHERA; URUGUAY. |
Asunto categoría : |
-- |
Marc : |
LEADER 02836naa a2200325 a 4500 001 1061285 005 2021-01-27 008 2020 bl uuuu u00u1 u #d 024 7 $adoi.org/10.3168/jds.2020-18177$2DOI 100 1 $aSCHILD, C. 245 $aA survey of management practices that influence calf welfare and an estimation of the annual calf mortality risk in pastured dairy herds in Uruguay.$h[electronic resource] 260 $c2020 500 $aArticle history: Received January 8, 2020/Accepted May 26, 2020. Corresponding authors: cschild@inia.org.uy and fgiannitti@inia.org.uy. 520 $aABSTRACT: Despite the importance of dairy farming in Uruguay,little information on dairy systems in this country is available in the scientific literature, and management practices that influence calf welfare at the herd level have not been explored. The aims of this study were to (1) describe the prepartum and calf-rearing systems, as well as the management practices that may influence calf welfare in pastured dairy herds in Uruguay, (2) estimate the annual calf mortality risk from birth to weaning, and (3) identify the primary clinical disease syndromes shown by the calves before death. A survey comprising a farm visit and a questionnaire was conducted on 225 randomly selected dairies with >30 milking cows, in 3 strata (31?99, 100?299, and ?300 milking cows) of 6 Uruguayan departments where dairies are concentrated. Retrospective information from July 2013 to June 2014 was collected. A descriptive analysis was performed and results were inferred into the national dairy cattle population. Several management practices that could contribute to poor calf welfare were identified in a large proportion of farms. The annual calf mortality risk (calves that died between birth and weaning/calves born death or alive × 100, n = 149 farms) was 15.2%. Age at weaning averaged 75 d. Farmers reported that the most common clinical syndromes were diarrhea and respiratory disease in 85.2% and 47.5% of the farms, respectively. There was no continuous veterinary advice in 61.3% of the farms, 20.0% lacked data records, 38.5% had poor drainage in the prepartum area with waterlogging after rainfall, 52.1% monitored the prepartum area ?2 times per day 650 $aPRODUCCION LECHERA 650 $aURUGUAY 653 $aBINESTAR DE LOS TERNEROS 653 $aCALF MORTALITY 653 $aCALF WELFARE 653 $aDAIRY FARMING 653 $aENCUESTA 653 $aMORTALIDAD DE TERNEROS 653 $aPLATAFORMA SALUD ANIMAL 653 $aSURVEY 700 1 $aCAFFARENA, D. 700 1 $aGIL, A. 700 1 $aSANCHEZ, J. 700 1 $aRIET-CORREA, F. 700 1 $aGIANNITTI, F. 773 $tJournal of Dairy Science, Volume 103, Issue 10, pag. 9418-9429, October 2020. Doi: https://doi.org/10.3168/jds.2020-18177
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|